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QCE Biology Units 3&4 — Paper 2 Question and Response Book

QCE Biology Units 3&4 — Paper 2 Question and Response Book — Free Online Pack 0

Read QCE Biology Units 3&4 — Paper 2 Question and Response Book online for free, including every question, worked solution, marking note and diagnostic action. No public PDF download or checkout is provided.

QCE Units 3&4 External Examination 2026 Edition - Pack 0 v2.0
Updated 8 Oct 2026

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Syllabus alignment: Biology General senior syllabus — 2025 v1.3.

Use this free 2026 pack now to work through practice questions and solutions ahead of the 2027 release. This is a 2026 edition; alignment to the 2027 course and assessment requirements has not yet been verified.

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  • Paper 1 Mon 2 Nov 2026 AM · scheduled 9:00 am Upcoming7 days left
  • Paper 2 Mon 2 Nov 2026 PM · scheduled 12:30 pm Upcoming7 days left

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QCE Biology Units 3&4 — Paper 2 Question and Response Book

11 questions

52 marks

Reading: 5 minutes perusal · Writing: 90 minutesFree online paper · no PDF download or checkout

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What Pack 0 covers

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Covered in this pack

  • QCAA Biology General senior syllabus 2025 v1.3 - Unit 3 Topic 1 - Biodiversity and populations 25 marks · 10 questions
  • QCAA Biology General senior syllabus 2025 v1.3 - Unit 3 Topic 2 - Functioning ecosystems and succession 25 marks · 9 questions
  • QCAA Biology General senior syllabus 2025 v1.3 - Unit 4 Topic 1 - Genetics and heredity 25 marks · 9 questions
  • QCAA Biology General senior syllabus 2025 v1.3 - Unit 4 Topic 2 - Continuity of life on Earth 26 marks · 9 questions

Read QCE Biology Units 3&4 — Paper 2 Question and Response Book online

Skill Align

Skill Align QCE Biology - Free Online Pack 0 Paper 2

An independently authored QCE Biology practice paper with worked answers and question-specific marking guidance.

Paper
Paper 2 Question and Response Book
Reading
5 minutes perusal
Writing
90 minutes
Assessment
52 marks

A QCAA-approved scientific or graphics calculator may be used. Notes and other unauthorised materials are not permitted.

Short-response questions

Questions 1-11 are short response. Answer all parts, show calculations and justify conclusions with the supplied biological evidence.

Question 1

3 marks
Stimulus

A reserve has wet gullies covering 20% of its area and dry slopes covering 80%. A rare orchid may occur in either habitat. Fifty equal-sized quadrats will be allocated in proportion to habitat area and positioned randomly within each habitat.

Use the following stimulus to answer all parts of this question.
(a) 3 marks
Apply proportional stratified sampling to determine the allocation and state the shared search condition.

Question 2

6 marks
Stimulus

Cassowary signs per kilometre along six equal-effort transects from the forest edge inward are 1, 2, 4, 7, 8 and 8. All transects are in one reserve, and sign persistence and visibility may vary with vegetation density.

Use the following stimulus to answer all parts of this question.
Graph Preview
12345686420Distance class from edgeSigns per kilometre
(a) 2 marks
Describe the spatial pattern in the observations.
(b) 4 marks
Interpret how strongly the observations support edge avoidance.

Question 3

6 marks
Stimulus

The pedigree shows a hypothetical human trait across three generations. Assume one gene controls the trait, genotypes always produce their expected phenotypes, and no new mutations or environmental effects change the recorded phenotypes. Females are XX and males are XY. Consider only autosomal dominant, autosomal recessive, X-linked dominant and X-linked recessive models. Birth outcomes are independent.

Use the following stimulus to answer all parts of this question.
Diagram Preview
Family pedigreeI-1I-2II-1II-2II-3III-1III-2Square: male; circle: female Filled: affected; unfilled: unaffected
(a) 3 marks
Infer the inheritance pattern and justify it using two different family relationships in the pedigree.
(b) 3 marks
Use A for the allele associated with the trait and a for the other allele. Determine the genotypes of II-1 and II-2, then calculate the probability that their next two children will both be unaffected. Show your reasoning.

Question 4

3 marks
Stimulus

Leaf litter falls onto a rainforest floor and is consumed by detritivores before microorganisms complete decomposition.

Use the following stimulus to answer all parts of this question.
(a) 3 marks
Explain the carbon transfers involved.

Question 5

9 marks
Stimulus

A canyon separated one frog population into eastern and western populations. Calls now differ between the populations, experimental crosses produce 22% viable offspring compared with 88% within-population crosses, and conserved DNA sequences differ more between than within populations.

Use the following stimulus to answer all parts of this question.
(a) 3 marks
Explain how the canyon can influence gene flow and allele frequencies.
(b) 3 marks
Analyse the call, crossing and sequence evidence for divergence.
(c) 3 marks
State three limitations of the species-status inference.

Question 6

4 marks
Stimulus

A purple-flowered plant of unknown genotype is crossed with a homozygous recessive white-flowered plant. Offspring are 51 purple and 49 white.

Use the following stimulus to answer all parts of this question.
(a) 2 marks
Determine the unknown genotype.
(b) 2 marks
Explain why a larger sample would increase the reliability of the conclusion.

Question 7

3 marks
Stimulus

Before a marine heatwave, allele H had frequency 0.27 in a snail population. Among surviving breeders its frequency was 0.48; 64% of H carriers survived compared with 29% of non-carriers.

Use the following stimulus to answer all parts of this question.
(a) 3 marks
Analyse the effect of the heatwave on allele H.

Question 8

2 marks
Stimulus

A frog is heard on 30% of single night surveys, but repeated surveys show it occupies 60% of wetlands.

Use the following stimulus to answer all parts of this question.
(a) 2 marks
Explain why the single-survey estimate is lower.

Question 9

2 marks
Stimulus

A fungus receives carbohydrates from tree roots and increases the roots' access to phosphate.

Use the following stimulus to answer all parts of this question.
(a) 2 marks
Classify and justify the interaction.

Question 10

10 marks
Stimulus

Independently replicated aquaria containing equal seagrass biomass are randomly assigned to 20%, 50% or 90% surface light for six weeks and rotated among bench positions. Dissolved nutrients, temperature and salinity are held constant. Mean biomass changes are -18%, +4% and +21%, respectively. The supplied measurements also include photosynthetic oxygen production, respiration, leaf area and final dry biomass.

Use the following stimulus to answer all parts of this question.
Graph Preview
2050903020100Surface light available (%)Mean biomass change (%)
(a) 3 marks
Interpret the treatment pattern.
(b) 3 marks
Analyse how the stated controls affect the strength of the light-treatment inference.
(c) 4 marks
Analyse how the supplied process and outcome measurements support a bounded interpretation.

Question 11

4 marks
Stimulus

PCR products from a regulatory locus are cut with an enzyme. Genotype LL gives a 420 bp band, ll gives 260 bp and 160 bp bands, and Ll gives all three bands. A DNA ladder and known LL, Ll and ll samples resolve at their expected positions, while a no-template lane has no band.

Use the following stimulus to answer all parts of this question.
(a) 2 marks
Identify the genotype of a sample with bands at 420 bp, 260 bp and 160 bp.
(b) 2 marks
Analyse how the supplied controls affect confidence in the genotype call.

QCE and QCAA external assessments are administered by the Queensland Curriculum and Assessment Authority (QCAA). Skill Align is an independent publisher and is not affiliated with, authorised by, sponsored by, approved by, or endorsed by QCAA or the Queensland Government.

Copyright (c) 2026 Skill Align. Free for personal, non-commercial online viewing at https://skillalign.au. You may share the Skill Align page link. Except as permitted by law or with Skill Align's prior written permission, the pack itself must not be resold, copied, redistributed, republished, automatically extracted, or uploaded to a question bank. Every question, dataset, answer and marking description in this pack is original Skill Align content.

Worked Solutions And Marking Guide

General marking principles

  • Award each listed mark independently when the required biological evidence is demonstrated.
  • Accept scientifically accurate equivalent wording and logically equivalent calculations.
  • Do not award an unsupported causal claim where the evidence establishes only an association.

Question 1

(a) - Treat wet gullies and dry slopes as separate strata. - Allocate 10 quadrats randomly within gullies and 40 randomly within slopes in proportion to area. - Use the same quadrat size and search rule so density estimates are comparable.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Treat wet gullies and dry slopes as separate strata.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Allocate 10 quadrats randomly within gullies and 40 randomly within slopes in proportion to area.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Use the same quadrat size and search rule so density estimates are comparable.

Question 2

(a) - Signs increase from 1 near the edge to 8 on the two deepest transects. - The increase levels off between the fifth and sixth transects.

(b) - The pattern is consistent with greater cassowary use of interior habitat. - Signs are an indirect measure, and variation in persistence or visibility can affect recorded counts. - Six transects from one reserve do not establish a general population response. - The evidence supports an association within the sampled reserve but does not prove that forest edges cause avoidance.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Signs increase from 1 near the edge to 8 on the two deepest transects.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The increase levels off between the fifth and sixth transects.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: The pattern is consistent with greater cassowary use of interior habitat.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: Signs are an indirect measure, and variation in persistence or visibility can affect recorded counts.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: Six transects from one reserve do not establish a general population response.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: The evidence supports an association within the sampled reserve but does not prove that forest edges cause avoidance.

Question 3

(a) - The pattern is autosomal dominant under the stated models and assumptions. - Affected parents I-1 and I-2 have unaffected daughter II-3; two affected parents would produce only affected children under either recessive model. - Affected father I-1 has unaffected daughter II-3; an X-linked dominant father would pass the dominant allele to every daughter, excluding that model.

(b) - II-2 is aa; II-1 is Aa because unaffected child III-2 is aa and must receive a from each parent. - For Aa × aa, each child has probability 1/2 of being aa and unaffected, and 1/2 of being Aa and affected. - Independent births give a probability of (1/2) × (1/2) = 1/4 for both of the next two children being unaffected; the previous children's outcomes do not change this probability.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The pattern is autosomal dominant under the stated models and assumptions.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Affected parents I-1 and I-2 have unaffected daughter II-3; two affected parents would produce only affected children under either recessive model.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Affected father I-1 has unaffected daughter II-3; an X-linked dominant father would pass the dominant allele to every daughter, excluding that model.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: II-2 is aa; II-1 is Aa because unaffected child III-2 is aa and must receive a from each parent.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: For Aa × aa, each child has probability 1/2 of being aa and unaffected, and 1/2 of being Aa and affected.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: Independent births give a probability of (1/2) × (1/2) = 1/4 for both of the next two children being unaffected; the previous children's outcomes do not change this probability.

Question 4

(a) - Detritivores ingest organic carbon and transfer some into their biomass. - Detritivore and microbial respiration return carbon dioxide to the atmosphere. - Unassimilated and dead material remains available to decomposers or soil carbon pools.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Detritivores ingest organic carbon and transfer some into their biomass.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Detritivore and microbial respiration return carbon dioxide to the atmosphere.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Unassimilated and dead material remains available to decomposers or soil carbon pools.

Question 5

(a) - The canyon geographically isolates the populations and reduces movement of alleles between them. - Mutation, genetic drift and different selection pressures can then change allele frequencies independently. - Continued reduction in gene flow permits genetic differences to accumulate across generations.

(b) - Different calls can reduce mating between eastern and western frogs. - Lower viability of between-population offspring provides evidence of reproductive isolation. - Greater between-population sequence differences support genetic divergence rather than a call difference alone.

(c) - Experimental crosses may not represent mate choice or offspring survival in the wild. - The number and geographic coverage of genetic samples are not stated, so the sequence comparison may not represent population-wide genetic variation. - Current movement across the canyon and persistence of the differences over time are not established.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The canyon geographically isolates the populations and reduces movement of alleles between them.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Mutation, genetic drift and different selection pressures can then change allele frequencies independently.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Continued reduction in gene flow permits genetic differences to accumulate across generations.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: Different calls can reduce mating between eastern and western frogs.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: Lower viability of between-population offspring provides evidence of reproductive isolation.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: Greater between-population sequence differences support genetic divergence rather than a call difference alone.

Part c (1 mark)

Awards one mark for accurately establishing this question-specific point: Experimental crosses may not represent mate choice or offspring survival in the wild.

Part c (1 mark)

Awards one mark for accurately establishing this question-specific point: The number and geographic coverage of genetic samples are not stated, so the sequence comparison may not represent population-wide genetic variation.

Part c (1 mark)

Awards one mark for accurately establishing this question-specific point: Current movement across the canyon and persistence of the differences over time are not established.

Question 6

(a) - The approximately 1:1 phenotype ratio is expected from Pp × pp. - The purple parent is therefore heterozygous Pp.

(b) - Random segregation causes sampling variation around the expected ratio. - A larger offspring count reduces proportional sampling error but does not make the genetic model immune to other explanations.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The approximately 1:1 phenotype ratio is expected from Pp × pp.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The purple parent is therefore heterozygous Pp.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: Random segregation causes sampling variation around the expected ratio.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: A larger offspring count reduces proportional sampling error but does not make the genetic model immune to other explanations.

Question 7

(a) - The heatwave is a selection pressure because survival differs between the stated genotypes. - Higher survival of H carriers and the rise from 0.27 to 0.48 support positive selection for H in this event. - One event does not establish that H will remain advantageous under other conditions or continue increasing.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The heatwave is a selection pressure because survival differs between the stated genotypes.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Higher survival of H carriers and the rise from 0.27 to 0.48 support positive selection for H in this event.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: One event does not establish that H will remain advantageous under other conditions or continue increasing.

Question 8

(a) - An occupied wetland may yield no call during one survey, creating false absences. - Repeated visits allow occupancy to be estimated separately from imperfect detection.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: An occupied wetland may yield no call during one survey, creating false absences.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Repeated visits allow occupancy to be estimated separately from imperfect detection.

Question 9

(a) - The interaction is mutualism because both species gain a resource-related benefit. - The fungus gains organic carbon while the tree gains improved mineral acquisition.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The interaction is mutualism because both species gain a resource-related benefit.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The fungus gains organic carbon while the tree gains improved mineral acquisition.

Question 10

(a) - Biomass declined at 20% light, changed little at 50% light and increased most at 90% light. - Within the tested range, greater light availability is associated with greater net biomass gain. - The result is consistent with light limiting photosynthetic production in the low-light treatment.

(b) - Equal starting biomass and controlled abiotic conditions make light the main stated treatment difference. - Independent aquaria provide replicate experimental units rather than treating shoots within one aquarium as independent. - Random assignment and rotation reduce systematic allocation and bench-position effects.

(c) - Photosynthetic oxygen production measures a process response to the light treatment. - Respiration data distinguish gross production from net production. - Leaf area and final dry biomass quantify biological outcomes using consistent endpoints. - The six-week aquarium result still does not establish responses across all field turbidity and duration ranges.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Biomass declined at 20% light, changed little at 50% light and increased most at 90% light.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: Within the tested range, greater light availability is associated with greater net biomass gain.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The result is consistent with light limiting photosynthetic production in the low-light treatment.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: Equal starting biomass and controlled abiotic conditions make light the main stated treatment difference.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: Independent aquaria provide replicate experimental units rather than treating shoots within one aquarium as independent.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: Random assignment and rotation reduce systematic allocation and bench-position effects.

Part c (1 mark)

Awards one mark for accurately establishing this question-specific point: Photosynthetic oxygen production measures a process response to the light treatment.

Part c (1 mark)

Awards one mark for accurately establishing this question-specific point: Respiration data distinguish gross production from net production.

Part c (1 mark)

Awards one mark for accurately establishing this question-specific point: Leaf area and final dry biomass quantify biological outcomes using consistent endpoints.

Part c (1 mark)

Awards one mark for accurately establishing this question-specific point: The six-week aquarium result still does not establish responses across all field turbidity and duration ranges.

Question 11

(a) - The sample is heterozygous Ll because it contains the uncut L fragment and both l restriction fragments. - The fragment sizes are consistent because 260 bp + 160 bp = 420 bp.

(b) - The ladder and known genotypes support fragment-size identification and show the amplification and digestion pattern worked as expected. - The clear no-template lane reduces a contamination explanation but does not by itself prove complete digestion in every sample.

Detailed marking criteria

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The sample is heterozygous Ll because it contains the uncut L fragment and both l restriction fragments.

Part a (1 mark)

Awards one mark for accurately establishing this question-specific point: The fragment sizes are consistent because 260 bp + 160 bp = 420 bp.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: The ladder and known genotypes support fragment-size identification and show the amplification and digestion pattern worked as expected.

Part b (1 mark)

Awards one mark for accurately establishing this question-specific point: The clear no-template lane reduces a contamination explanation but does not by itself prove complete digestion in every sample.

Diagnostic Checklist

TopicQuestionsMarksMarks LostAction
Unit 3 Topic 1 - Biodiversity and populations Q1, Q2, Q8 11 ___ Review classification, sampling, population estimates, diversity indices and evidence limits.
Unit 3 Topic 2 - Functioning ecosystems and succession Q4, Q9, Q10 15 ___ Review energy transfer, biogeochemical cycling, species interactions, disturbance and succession.
Unit 4 Topic 1 - Genetics and heredity Q3, Q6, Q11 14 ___ Review DNA, gene expression, meiosis, inheritance, biotechnology and quantitative genetic reasoning.
Unit 4 Topic 2 - Continuity of life on Earth Q5, Q7 12 ___ Review mutation, natural selection, speciation, phylogeny and converging evolutionary evidence.

What is included

Paper 1 Combined Independent Practice Book questions (49 marks)

Paper 2 Question and Response Book questions (52 marks)

Worked solutions and marking guidance shown online

Diagnostic checklist shown online

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How to use this pack

  1. Attempt: Open the complete paper online and work under the timing and conditions shown on this page.
  2. Mark: Use the supplied worked solutions or response support and marking guidance to check answers and method.
  3. Review: Use the supplied diagnostic or review support to identify the next areas for revision.

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  • QCE Biology Units 3&4
  • 2026 edition · Pack 0 · v2.0
  • 2 online papers
  • Free online view · no checkout or PDF download
  • Free to view online
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Questions about this exam pack

What is included in Biology Free Online - Pack 0?

Pack 0 includes 2 full-length showcase papers, worked solutions, marking guidance and diagnostic checklists, all shown online.

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Yes. Pack 0 can be read online without checkout or a monthly subscription.

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No. Pack 0 is intentionally online-only and no downloadable PDF is provided.

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No. The questions are original Skill Align material. Skill Align is independent and is not affiliated with, authorised by, sponsored by, approved by, or endorsed by any state assessment authority.

Independent practice resource

Queensland Certificate of Education (QCE) subjects and external assessments are administered by the Queensland Curriculum and Assessment Authority (QCAA). Skill Align is an independent publisher and is not affiliated with, authorised by, sponsored by, approved by, or endorsed by QCAA or the Queensland Government.

Each exam pack is listed with a pack label so buyers can distinguish separate original products in the same course series. Pack numbers do not indicate difficulty or a required completion order.

2026 exam timetable reference View QCAA 2026 timetable
  • Paper 1 Mon 2 Nov 2026 AM · scheduled 9:00 am Upcoming7 days left
  • Paper 2 Mon 2 Nov 2026 PM · scheduled 12:30 pm Upcoming7 days left

QCAA schedules morning and afternoon sessions at 9:00 am and 12:30 pm. Your school may start within the permitted 15-minute window; check its final directions and any clash allocation.